Cytotoxicity and Inflammatory Mediators Release by Macrophages Exposed to Real Seal XT and Sealapex Xpress

Abstract This study evaluated the cytotoxicity of Sealapex Xpress and Real Seal XT their effect on macrophage activation. J774.1 macrophages were incubated with (0.1, 1.0, 10 mg/mL) for 24 48 h. Cell viability was assessed by MTT assay activation measured pro- anti-inflammatory cytokine production using ELISA. Data analyzed one-way ANOVA Tukey’s post-test (a=0.05). not affected 0.1 or 1.0 mg/mL extracts at h (p>0.05), but significantly lower when cells exposed to both sealers (p<0.05). inhibited TNF-a, whereas induced TNF-a secretion IL-6 XT, IL-10. IL-4 detected in any group. In conclusion, had low toxicity differentially activated macrophages. Macrophage characterized inhibition induction IL-10, solely.